FIG 3.

EV71 2C does not influence the location of PP1c. HeLa cells were transfected with Flag-PP1α (A), Flag-PP1β (B), or Flag-PP1γ (C); cotransfected with 2C-HA and Flag-PP1α (A), Flag-PP1β (B), or Flag-PP1γ (C); or transfected with Flag-PP1α (A), Flag-PP1β (B), or Flag-PP1γ (C) and infected with EV71 (MOI = 5). After 24 h, immunofluorescence staining with the corresponding antibodies was performed, and the nuclei were subsequently stained with Hoechst 33258. After three washes (5 to 10 min each), the cells were observed using a 60× oil immersion objective. Fluorescent images were taken under a PerkinElmer UltraView VOX confocal microscope. Each image is representative of the findings for the majority of cells observed in several fields. The results shown are representative of those from two independent experiments.