FIG 3.

A2(91G) infection-recruited CD8 T cells exhibit a skewed CD127^hi KLRG1^lo phenotype and have a larger recall response. (A) At days 8 and 84 p.i., spleen cells were surface stained with D^bLT359 tetramers and MAbs to KLRG1 and CD127. (Left) Contour plots representative of 3 to 5 mice per time point and three independent experiments; (right) frequencies of LT359-specific CD8 T cells with the MPEC (KLRG1^lo CD127^hi), DPEC (KLRG1^hi CD127^hi), SLEC (KLRG1^hi CD127^lo), and EEC (KLRG1^lo CD127^lo) phenotypes. Values indicate means and SEM. *, P < 0.05; **, P < 0.005. (B) At 80 days p.i. with A2 or A2(91G), B6 mice were challenged with rVSV-LT359. (Left) Representative mean frequencies of D^bLT359 tetramer⁺ CD44⁺ CD8 T cells in blood before and 5 days after challenge; (right) pre- versus postchallenge fold expansion, with each dot corresponding to an individual mouse and horizontal lines indicating means ± SEM. Data were collected from two independent experiments (n = 4 or 5 mice for each group).