Fig. 3.

Hesperidin protects against UVB-induced oxidative damage to cellular macromolecules. HaCaT cells were treated with hesperidin (50 μM) for 1 h and then exposed to UVB radiation. (A) DNA damage was measured by the alkaline comet assay. Representative images and the percentage of total DNA fluorescence in comet tails are shown. (B) After 24 h of incubation, DNA fragmentation was assessed by colorimetric determination of BrdU-labeled DNA fragments. (C) Lipid peroxidation was measured by detecting the level of 8-isoprostane secreted into the culture medium. (D) Protein oxidation was assayed by detecting the level of protein carbonyls. *^,#Significantly different from control and UVB-irradiated cells, respectively (p<0.05).