Figure 4. Cotranslational miRNA-mediated decay involves mRNA deadenylation.
(A) Schematic depiction of the polyA tail length assay to measure the length of a polyA tail. (B) PolyA tail lengths for the indicated mRNAs. Tail lengths were determined for each mRNA in total RNA and pooled polysome gradient fractions as indicated. This assay does not have the sensitivity to detect the endogenous hid 3’ end. (C) The deadenylated species of reaper reporter and endogenous reaper were cloned and sequenced. AATAAA: polyA signal. Genomic DNA sequences are shown in black while the reverse primer is shown in purple.