Figure 6. Development of topographies.

The position of the centers of mass of the labelling in layers I and III of PrS, layers V-VI of PrS and PaS and layers V-VI of MEC and LEC is plotted (see also Figure 6—figure supplement 2). Each dot is color coded with respect to the rostrocaudal (A) or dorsoventral position (B) of the injections in RSC (rostral half; blue, caudal half; red, A29; yellow, A30; green). Left column; animals aged P1-6, middle column; animals aged P7-13, right column; animals aged P14-28. Age is not related to the dorsoventral position of the plexus (PrS LI-III: p=0.876; PRS and PaS LV-VI; p=0.187; EC LV-VI: p=0.198) or the transverse position of the plexus (PrS LI-III: p=0.641; PrS and PaS LV-VI; p=0.325; EC LV-VI: p=0.402). Multiple regression was used for all statistical tests (Figure 5—source data 1–12). For flatmaps of the projection patterns see Figure 6—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.13925.023

Figure 6—figure supplement 1. Flatmaps of projection patterns of different age groups.

All injections were subdivided into three groups based on the age of the injected animal. 1^st column shows flatmaps of averaged labeling patterns in animals injected during the 1^st postnatal week, 2^nd column shows flatmaps of animals injected during the 2^nd postnatal week, 3^rd column shows flatmaps of animals injected during the 3^rd postnatal week or later and the 4^th column contains animals across all ages. The 1^st row shows flatmaps of labeling patterns independent of the location of the injection. Numbers under the flatmap depict the number of experiments in each group. The 2^nd row shows flatmaps of the labeling patterns observed after injections in A30, while the 3^rd row shows flatmaps of the labeling patterns observed after injections in A29. In row 3 and 4 the numbers under the flatmap depict the number of injections in rostral quarter of RSC + the number of injections in the intermediate-rostral quarter of RSC + the number of injections in the intermediate-caudal quarter of RSC + the number of injections in the caudal quarter of RSC. The 4^th row shows flatmaps of the labeling pattern observed after injections in the rostral half (R) of RSC while the 5^th row shows injections in the caudal half (C) of RSC. In rows 4 and 5 the numbers under the flatmap depict the number of injections in A29 + the number of injections in A30 in the respective group. Dorsal, ventral, proximal (prox), distal (dist), medial (med), lateral (lat) indicates dorsoventral and transverse axis of the flatmaps.

DOI: http://dx.doi.org/10.7554/eLife.13925.024

Figure 6—figure supplement 2. Development of topographies.

(A–C) Centers of mass of the labeled plexus after each injection were calculated for PrS layers I and III (A), PrS and PaS layers V-VI (B) and MEC and LEC layers V-VI (C). In the plots each injection is plotted according to the age of the animal and the rostrocaudal position in RSC and color coded according to the dorsal (blue), ventral (red) position of the center of mass of the projection in PHR (see color maps in bottom left of each figure). (D–F) Centers of mass of the labeled plexus after each injection were calculated for PrS layers I and III (D), PrS and PaS layers V-VI (E) and MEC and LEC layers V-VI (F). In the plots each injection is plotted according to the age of the animal and the dorsoventral position in RSC and color coded according to the proximal/lateral (yellow) and distal/medial (green) position of the center of mass of the projection in PHR (see color maps in top right of each figure).

DOI: http://dx.doi.org/10.7554/eLife.13925.025