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Effect of days in vitro on the percentage of type 1 astrocytes expressing κ opioid receptor (KA8) immunoreactivity.
| Days in Vitro | Appearance of cultures | Astrocytes expressing κ receptors (%) | Astrocyte density (Cells/0.1 mm2) |
|---|---|---|---|
|
| |||
| 6–7 | Subconfluent | 52.2 ± 3.0 | 6.8 ± 1.0 |
| 14 | Confluent | 13.6 ± 4.0* | 25.6 ± 3.1* |
Mean ±SEM of 7 cultures; each culture consisted of independent samples of cells from separate mice.
P< 0.01 vs. cultures at 6 –7 days in vitro.
