FIG. 8.

(a) Translocation resistive pulse signals of a 50-50 mixture of 22 b ssDNA and dsDNA after 5 min and 10 min. Average ssDNA translocation time is about 100 ms, compared to the average dsDNA translocation time of 1 ms. The distinctly different translocation times allow easy identification of the two events. The longer ssDNA translocation events initiate only after 10 min. (b) STED super-resolution microcopy image at 30 min shows that this delay of ssDNA translocation is due to an adsorption step onto the membrane surface before migration into the tip (see 100 nm depletion ring in the image).