Figure 1.

Whrn Isoform Expression

(A) Diagram of Whrn exons showing deletions in Whrn^wi/wi (orange) and Whrn^tm1b/tm1b (blue), with regions coding for WHRN domains colored (green, PDZ1; blue, PDZ2; red, PDZ3; purple, proline-rich domain). Vertical arrows mark target region of antibodies PB584 and PB595. Horizontal arrows mark locations of primers.

(B) Ensembl predictions of Whrn isoforms. Narrow boxes indicate UTRs and wider boxes protein-coding regions. Left: isoform names used in this paper. Right: Ensembl transcript IDs. ENSMUST00000155058 is classified as a retained intron transcript but was recently found in the inner ear (Mathur et al., 2015b) and results in a protein sequence identical to transcript ENSMUST00000119294, one of the WHRN-S isoforms, when translated. (GenBank accession numbers provided in Table S1). Primers used to detect each isoform are shown on the relevant isoform.

(C–E) Transcripts detected in wild-type (C), Whrn^tm1b/tm1b, (D) and Whrn^wi/wi, and (E) inner ears. Black lines indicate splice junctions observed by sequencing. Grey lines indicate splice junctions inferred by presence of the isoform, but not observed by sequencing.

See also Figure S1.