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. 2016 Mar 15;6(4):843–858.

Figure 4.

Figure 4

DAVLBH suppressed VEGF/VEGFR2 and Gas6/Axl signaling pathways. DAVLBH inhibited VEGFR2, Axl, Akt and ERK in (A) VEGF-treated HUVECs and (B) Gas6-treated HUVECs. HUVECs were pre-treated with various concentrations of DAVLBH for 4 h and then stimulated with VEGF or Gas6 for 1 h. Protein was collected and subjected to western blot analysis. (C) DAVLBH inhibited the VEGF- and Gas6-induced activation of Rac1 and Cdc42. HUVECs were starved with serum-free ECM and treated with different concentrations of DAVLBH for 4 h and then stimulated with VEGF or Gas6 for 1 h. The cells were then lysed, and active Rac1 and Cdc42 were pulled down and applied to western blotting.