Figure 5.

Methylated DNA and histone H3 acetylation in transformed and normal cells under acidosis: (A) Methylation profile of genomic DNA was screened by COBRA for LINE1 in OS cells, Fb, and MSCs under neutral (N) and acidic (A) conditions. Representative image (upper panel), and bar graph (lower panel) of the densitometric analysis (mean ± SE, *p < 0.05, n = 3). DNA from NEC8, a testicular embryonal carcinoma, was used as an unmethylated control for LINE1. (B) HDAC1 and acetylated histone H3 (Ac-H3) were evaluated by immunoblots in OS cells, Fb, and MSCs under both neutral (N) and acidic (A) conditions using specific antibodies. The graphs (lower panel) show the densitometric analyses using α-tubulin as control (mean ± SE, *p < 0.05, n = 4).