Table 1.

Metabolites with lower intracellular concentration in osteosarcoma cells than in normal fibroblasts under acidosis analysed by CE-TOFMS

Compound name	Category	OS		Fb		OS/Fb	P-value
		Mean [pmol/109 cells]	SD	Mean [pmol/109 cells]	SD
Dihydroxyacetone phosphate	Glycolysis	4.65	2.86	15.65	0.33	0.30	0.0295*
Phosphocreatine	Protein metabolism	34.40	9.01	194.80	5.01	0.18	0.0003**
Glu	Glycogenic amino acid	237.14	123.82	642.46	76.19	0.37	0.0424*
Argininosuccinic acid	Uremic toxin	0.98	0.26	5.30	0.53	0.19	0.0398*
ATP	Purine bases	807.41	217.89	1618.76	100.12	0.50	0.0218*
GTP	Purine bases	34.66	8.57	100.27	7.71	0.35	0.0169*
CTP	Pyrimidine bases	67.44	17.70	118.23	6.50	0.57	0.0407*
UTP	Pyrimidine bases	220.87	152.84	1057.52	142.54	0.21	0.0379*
UDP-glucose, UDP-galactose	Carbohydrate metabolism	95.97	48.50	397.60	27.31	0.24	0.0066**
CMP-N-Acetylneuraminate	Nucleotide sugars	12.03	6.19	58.75	6.56	0.20	0.0308*
NAD+	Electron carrier	93.47	65.68	369.37	49.97	0.25	0.0373*
N 8-Acetylspermidine	Histone acetylation	12.94	10.51	429.06	9.76	0.03	0.0006**
Pelargonic acid	Fatty Acids	8.54	8.04	37.42	5.92	0.23	0.0489*
Decanoic acid	Fatty Acids	4.10	2.90	19.12	0.43	0.21	0.0150*

Welch’s t-test was used to evaluate the ratios of osteosarcomas (OS) vs. normal fibroblasts (Fb). In more details, all the data derived form OS cells (by including all the cell lines together) under acidosis were compared to all the data derived from Fb cells (by including all the different lots of cells together).

^*p < 0.05;

^**p < 0.01.