Echocardiographic assessment of knockout and control wild type mice. A. Echocardiography was performed before tamoxifen injection, 3 days after injection, 10 days after injection, 17 days after injection, 60 days after injection. B. Ejection fraction (EF%), fraction shortening (FS%), left ventricular mass (LV Mass [mg]), left ventricular end-diastolic volumes (LV vol [μl]) were calculated. C. Quantitative real-time PCR assay was carried out to examine the expression levels of fetal genes including A. ANF, B. BNP, and C. βMHC. n ≥ 6 in each group, *P < 0.05, **P < 0.01.