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. 2016 Apr 15;8(4):1826–1837.

Figure 5.

Figure 5

miR-544 targeted PARK7 by binding to its 3’-UTR. A. The correlation between miR-544 level and PARK7 mRNA level in 24 cases of glioma tissues was detected using real-time PCR. B. The sequence targeted by miR-544 in 3’-UTR of PARK7 protein (PARK7-3’UTR wt) and its corresponding mutant containing 4 mutated nucleotides (PARK7-3’UTR mut) were identified using bioinformatic analysis. C. The relative level of PARK7 mRNA was measured in U87-2M1 glioma cells transfected with miR-544 mimics or miR-control. D. The relative level of PARK7 mRNA was detected in SW1088 glioma cells transfected with miR-544 mimics or miR-control. E. The relative level of PARK7 mRNA was measured in U138 glioma cells transfected with anti-miR-544 or anti-control. F. The protein expression levels of PARK7 were detected in the cells transfected with the indicated RNA or DNA moleculars. G. U87-2M1 cells were co-transfected with miR-544 mimics and luciferase reporters containing either the predicted miRNA target site in PARK7 mRNA 3’-UTR or its corresponding mutant form. The cells co-transfected with miR-control and luciferase reporters was exploited as the control group. Error bars represent SEM. *, P < 0.05.