PBA inhibits the increase of BSCB permeability and improves locomotor functional recovery after SCI. After SCI, rat were treated with PBA and BSCB permeability was measured at 24 h post-SCI by using Evan’s Blue dye (n = 4/group). A. Representative whole spinal cords showing Evan’s Blue dye permeabilized into spinal cord at 24 h. B. Quantification of BSCB permeability data from A by software ImageJ. C. Quantification data of EB content of spinal cord (μg/g). D. Representative confocal images of an Evan’s Blue extravasation in each group at 24 h after SCI. E. Quantification of the fluorescence intensity of Evan’s Blue in each group. All data represent Mean values ± SEM, n =5. *P < 0.01 versus vehicle controls. F. The BBB scores of sham, SCI group and SCI rat treated with PBA group. *P < 0.05 versus the SCI group, and **P < 0.01 versus the SCI group, n = 6.