Table 1.
The values of the fraction of hemoglobin dimer γ, defined by Eq. (9); the observed rate constant of dissociation of hematin from methemoglobin k−H(obs), defined by Eq. (8); the equilibrium constant for reaction (Eq. (7)) K−H = k−H(obs)/kH computed using kH = 1 × 108 M−1s−1 for both dimers and tetramers [2,29]; and the equilibrium concentration of free heme Cheme, calculated using Eq. (10) for three values of CHb+.
|
CHb+
|
γ |
k−H(obs)
|
K−H
|
Cheme
|
|
|---|---|---|---|---|---|
| mg mL−1 | mM | 10−4s−1 | pM | nM | |
| 3.5 | 0.054 | 0.077 | 7.10 | 7.10 | 20 |
| 11.5 | 0.18 | 0.045 | 5.93 | 5.93 | 33 |
| 100 | 1.55 | 0.015 | 4.73 | 4.73 | 85 |