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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Leuk Res. 2013 Nov 5;38(4):430–434. doi: 10.1016/j.leukres.2013.10.026

Figure 1. Primary patient AML blasts do not express EGFR transcript or protein based on western immunoblotting and RT-PCR.

Figure 1

(A) Primary patient AML bone marrow samples were assessed by western immunoblotting for the expression of EGFR protein. An EGFR-expressing positive control HCC827, and an EGFR-non-expressing negative control, MV4-11, were included. Actin was evaluated as a loading control.

(B) Primary patient AML peripheral blood mononuclear cells were assessed in the same manner as in (A) for expression of EGFR protein.

(C) RT-PCR was performed to evaluate for EGFR transcript expression in primary patient AML cells. GAPDH was amplified as a control gene and an EGFR-expressing positive control, HCC827, and an EGFR-non-expressing negative control, MV4-11, were included.