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. Author manuscript; available in PMC: 2016 Nov 5.
Published in final edited form as: Nature. 2016 Apr 20;533(7601):115–119. doi: 10.1038/nature17950

Figure 3. Mice with LAP deficiencies display defective clearance of engulfed, dying cells, resulting in increased production of pro-inflammatory cytokines.

Figure 3

A–D. 1×107, PKH26-labeled UV-irradiated wild-type thymocytes were injected intravenously into indicated animals expressing GFP-LC3. (A, B) Apoptotic thymocytes in spleen, liver, and kidney of indicated animals measured by flow cytometry. (C, D) Indicated serum cytokines. Error bars represent standard deviation (n=4, *p < 0.001, **p < 0.05). E. 2×107, UV-irradiated wild-type thymocytes were injected intravenously six times over 8 weeks into indicated animals (aged 6 weeks). Serum anti-nuclear antibodies (ANA, Total Ig) and anti-dsDNA antibodies (Total Ig) are shown at 16 wks. Results are presented as ratio to average value prior to injection for each individual animal. Error bars represent standard error (n=4, **p < 0.05). Cre indicates LysM-Cre. The color scheme represents LAP-deficient, autophagy-deficient genotypes (green), autophagy-deficient, LAP-sufficient (red), and autophagy-sufficient, LAP-deficient (blue).