Extended Data Figure 5. Mice with LAP deficiencies display increased expression of the IFN signature but normal phagocytic capacity.

A. Wild-type and deficient littermates were co-housed and aged for 52 weeks at St. Jude Children’s Research Hospital (SJCRH). RNA was extracted from 52 week old spleens and analyzed for expression of genes associated with the IFN signature using Nanostring technology. Heat map of Nanostring counts from the top 26 regulated genes in the IFN signature are shown in triplicate per genotype. B. UV-irradiated wild-type thymocytes were stained with CellTrace Violet and co-cultured (5:1) with bone marrow-derived macrophages from wild-type and deficient genotypes for 45 minutes. Percent phagocytosis (% Cell Trace Violet) was quantified by flow cytometry (Singlets/GFP⁺ Cell Trace Violet⁺). C. Wild-type and deficient littermates were co-housed and aged for 52 weeks at St. Jude Children’s Research Hospital (SJCRH). Peritoneal macrophages were isolated after 3 days of intra-peritoneal injection of thioglycolate. UV-irradiated wild-type thymocytes were stained with CellTrace Violet and co-cultured (2:1) with peritoneal macrophages from wild-type and deficient genotypes for 1 hour. Phagocytic efficiency (Singlets/Cell Trace Violet⁺/F4/80⁺) was quantified by flow cytometry (% Cell Trace Violet). Error bars represent standard deviation. Data shown is representative of two independent experiments.