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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Neurobiol Dis. 2016 Mar 5;91:166–181. doi: 10.1016/j.nbd.2016.03.003

Figure 3.

Figure 3

VGLUT1/PSD95 close appositions on somata. A: Representative 1μm optical section through the soma of a layer V pyramidal cell from an undercut P21 rat perfused with 4% paraformaldehyde, and reacted for PSD95 (green), VGLUT1 (red) and NeuN (black soma). White circles: colocalization (yellow spots) of post-synaptic PSD95 and presynaptic VGLUT1 in the neuropil (bottom 2 circles) and at 4 presumptive excitatory somatic synapses. Arrow points to presumptive synapse on neighboring cell shown in ortho view in Fig. S2. B. Comparable section from control cell showing PSD95/VGLUT1 colocalizations are present in neuropil. White arrow points to rare possible synapse on soma (see Table 2). Apical dendrite down. Calibration in A for A–B