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. Author manuscript; available in PMC: 2017 May 5.
Published in final edited form as: Cell. 2016 Apr 7;165(4):936–948. doi: 10.1016/j.cell.2016.03.026

Figure 1. Glial KCC-3 controls AFD neuron receptive-ending shape.

Figure 1

(A) AMsh glia ensheathes multiple neurons. AFD (orange) also has a cilium (blue). Boxed region magnified in Figure 7. (B) Histogram depicting AFD defects in indicated genotypes. Numbers, animals scored. Error bars, SEM. ***p<0.0002. For transgenics, three independent lines were scored. Green text, glia expression; red text, AFD expression. Second bar, non-transgenic siblings (C,D) Fluorescence images of wild-type (C) and kcc-3(ok228) (D) AFD microvilli. Scale bar, 1µm. Arrow, dendritic shaft. Arrowhead, microvilli. (E) kcc-3 gene structure, deletions used are indicated by red and blue bars. Sequences not included in WormBase annotation, orange. (F) Histogram details as in Figure 1B. Time, hours post mid L4 stage. (G, H) Thermotaxis behavior assays for indicated genotype raised at 15°C (G) and 25°C (H). Animals in (G), 24 hours post mid-L4 stage. Animals in (H), 96 hours post mid-L4 stage. N, Number of animals. See also Figure S1 and S2.