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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Virology. 2016 Mar 19;493:52–59. doi: 10.1016/j.virol.2016.03.005

Figure 2. Class I MHC and costimulation mediate poly(I:C)-induced naïve T cell priming.

Figure 2

Congenic P14 cells were adoptively transferred into WT B6, TAP KO, or B7 KO mice prior to HBSS or poly(I:C) inoculation. Splenocytes were harvested one day later and stained directly ex vivo (A) or after 5 hour ICS stimulation with GP33 (B-C). Representative flow plots gated on CD44lo P14 CD8 T cells from HBSS- (shaded histograms) or poly(I:C)-treated (open histograms) mice expressing CD69, Eomes, and IRF4 (A) or TNF and IFNγ (B) are depicted. Frequencies of CD44lo IFNγ+ P14 T cells from WT B6 or B7 KO priming hosts stimulated in the presence of WT HBSS- or poly(I:C)-primed splenocytes (C) are graphed. Data are representative of at least 2-4 independent experiments with 2-4 mice per group.