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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Virology. 2016 Mar 19;493:52–59. doi: 10.1016/j.virol.2016.03.005

Figure 3. Primed naïve T cells elicit immediate effector functions in response to lower concentrations and affinity ligands.

Figure 3

Congenic P14 or OT-1 cells were transferred into WT B6 mice that were inoculated with HBSS (grey bars) or poly(I:C) (red bars). One day after inoculation, splenocytes were isolated and stimulated ex vivo with different concentrations of GP33 peptide (1000 nM, 250 nM, 64 nM, 16 nM or no peptide) (A-C) or different altered peptide ligands (OVA, Y3, T4, V4, G4, or no peptide) (D-F). The frequencies of CD44lo P14 (A-C) or OT-1 (D-F) cells that produced IFNγ (A, D), TNF (B, E), or granzyme B (C, F) are graphed. Data are representative of 2 individual experiments with 3-5 mice per group.