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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Virology. 2016 Mar 19;493:52–59. doi: 10.1016/j.virol.2016.03.005

Figure 4. Naïve T cells exhibit cytolytic capability in vivo after poly(I:C) priming.

Figure 4

Congenic P14 splenocytes were adoptively transferred into WT B6 mice that were inoculated with HBSS or poly(I:C). Peptide pulsed splenocytes were transferred into HBSS or poly(I:C) treated hosts for a 20 hour in vivo cytotoxicity assay. A) Representative histograms gated on donor splenocytes with the lowest concentration of CellTrace Violet representing no peptide-pulsed (Red), middle concentration, K3L peptide (Green), or highest concentration, GP33 peptide (Blue). B) Percent specific lysis was calculated for K3L- and GP33- specific cells. C) Representative flow plots gated on donor P14 cells expressing CD69 or CD62L from HBSS- and poly(I:C)-treated mice are shown. Correlation between % specific lysis and frequency of P14 cells expressing CD69 (D) or CD62L (E) is graphed. Data are representative of 2 individual experiments with n of 3-5 mice per group.