Figure 3. Effect of H2O2 injection on modulation of the antioxidant gene network in Amblyomma maculatum tissues.
Tick β-Actin was used as the reference gene for normalization. Transcriptional expression of genes encoding known selenoproteins (CAT, dual oxidase, superoxide dismutase and apoptosis marker genes caspase1, caspase2) in unfed A. maculatum midgut tissues (A) and salivary glands (B) following injection of either 3%, 5% or 7% H2O2 to assess the effects of induced oxidative stress. The gene expression values of all the genes except GSHR are statistically significant (P<0.05) when compared to the control. SG, salivary glands; MG, midgut.