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. Author manuscript; available in PMC: 2017 May 5.
Published in final edited form as: Cell. 2016 Apr 21;165(4):921–935. doi: 10.1016/j.cell.2016.04.001

Figure 5. Reduced microglia number and preservation of synaptic density in the ventral thalamus of Grn−/−;C1qa−/− mutant mice.

Figure 5

(A–H) Immunostains for Iba-1 in coronal sections of 12 month-old Grn+/+, Grn−/−, C1qa−/− and Grn−/−;C1qa−/− mouse brains at the level of anterior hippocampus. The square dotted boxes in panels A, C, E and G highlight the ventral thalamus, where the higher magnification images are obtained. Scale bar 500 μm in A and 50 μm in B. (I) Iba-1+ microglial density in the ventral thalamus of Grn+/+, Grn−/−, C1qa−/− and Grn−/−;C1qa−/− mouse brains at 2, 4, 7, 12 and 19 month-old. *** p < 0.005, **** p < 0.001, two-way ANOVA, n = 4 per genotype per age. (J–M) Confocal images of synaptophysin in 12 month-old Grn+/+, Grn−/−, C1qa−/− and Grn−/−;C1qa−/− brains. Scale bar 10 μm in J. (N) Synaptophysin density in Grn+/+, Grn−/−, C1qa−/− and Grn−/−;C1qa−/− brains at 2, 4, 7, 12 and 19 month-old. * p < 0.05, ** p < 0.01, *** p < 0.005, ns, not significant. Student’s t test, n = 4 per genotype per age.