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. 2016 Apr 24;2016:8727289. doi: 10.1155/2016/8727289

Figure 6.

Figure 6

Effect of HMW-HA, MMW-HA, LMW-HA, and U0126 on IL-8 mRNA transcript. The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), LMW-HA (100 μg/mL), and U0126 (25 μM) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 18 hrs to study IL-8 mRNA. mRNA levels were quantified by quantitative Real-Time PCR (see Materials and Methods for details). The bars represent the mean ± SD of 3 separate experiments. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.