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. 2016 Apr 25;4:e1971. doi: 10.7717/peerj.1971

Figure 1. Failed attempts to identify the PKC gene involved in operant self-learning.

Figure 1

Performance indices (PI) during a test period following an 8 min training session is reported. LEFT: Flies putatively mutant for PKC genes (PKC-53e, PKC-delta and PCK-InaC) performed well in the self-learning assay. RIGHT: Flies with RNAi constructs targeting PKC53e and PKC InaC were crossed to elav-Gal4;tub-Gal80ts or to CS females. RNAi was induced for two days before the experiment via a 32°heat-shock. While the construct targeting PKC InaC had no effect, the construct for PKC53e prevented self-learning formation even in absence of Gal4 driven expression, such that no firm conclusions can be drawn. Full genotypes of the flies tested are indicated below. CS × 53eRi_V: ;;UAS_PKC53eRNAi_27696/+. elavG4;tG80 × PKC53eRi_V: elavGal4/+;tubGal80ts/+;UAS_PKC53eRNAI_27696/+. elavG4;tG80 x PKCInacRi : elavGal4/+;tubGal80ts/+;UAS_PKCInacRNAI_2895/+. Data is shown as Tukey’s boxplots (median is the line surrounded by boxes representing quartiles) with a superposed violinplot. Asterisks indicate significant differences of the scores against 0, using a non-parametrical Wilcox test.