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. 2016 Apr 25;4:e1971. doi: 10.7717/peerj.1971

Figure 3. Flies with PKCi expression in motorneurons, are impaired in self-learning.

Figure 3

(A) Using OK371-Gal4 (expression in most glutamatergic neurons) or d42-Gal4 to drive PKCi expression was effective in preventing self-learning, while the control flies seem to learn, although the score of the d42Gal4 × CS control line was not statistically significantly positive. (B) Both D42-Gal4 and c380-GAL4 driving PKCi expression overlappingly in motorneurons, yield comparable inhibition of self-learning. (C) The use of the d42Gal4,chaGal80 double construct as a driver was effective in preventing self-learning, while the controls performed well. Heat-shock protocol was a 4-hour heat-shock at 35 °C. Full genotypes of the flies tested are indicated below. CS × T_PKCi: tubGal80ts/+; UAS-PKCi/+. d42G4 × T_PKCi : tubGal80ts/+; UAS-PKCi/d42-Gal4 . OK371G4 × T_PKCi : tubGal80ts/+; UAS-PKCi/+;OK371/+. d42G4 × CS : d42Gal4/+. OK371 × CS : OK371/+.c380G4 × T_PKCi : c380-Gal4/+. c380G4 × CS : c380Gal4/+; tubGal80ts/+; UAS-PKCi/+. d42G4,chaG80 × CS : d42-Gal4, cha-Gal80/+. d42G4,chaG80 × T_PKCi : tubGal80ts/+; UAS-PKCi/d42-Gal4, cha-Gal80 . CS × T_PKCi : tubGal80ts/+; UAS-PKCi/+. Data is shown as Tukey’s boxplots (median is the line surrounded by boxes representing quartiles) with a superposed violinplot. Asterisks indicate significant differences of the scores against 0, using a non-parametrical Wilcox test.