. Author manuscript; available in PMC: 2017 Feb 21.

Published in final edited form as: Org Biomol Chem. 2016 Jan 21;14(7):2186–2190. doi: 10.1039/c5ob02609a

Table 1.

Kinetic data for sirtuins against different fluorogenic substrates.

	SIRT1			SIRT2			SIRT3

	K_m (μM)	k_cat (10⁻³s ⁻¹)	k_cat/K_m (s⁻¹M⁻¹)	K_m (μM)	k_cat (10⁻³s⁻¹)	k_cat/K_m (s⁻¹M⁻¹)	K_m (μM)	k_cat (10⁻³s⁻¹)	k_cat/K_m (s⁻¹M⁻¹)
3	> 333^a	ND^a	1.2 × 10²	> 333^a^,^b	ND^a	18.8	> 333^a^,^b	ND^a	7.3
4	35.3 ± 4.5	17 ± 1.1	4.8 × 10²	< 0.50^c	10 ± 0.5	> 2.0 × 10⁴	3.6 ± 1.2	2.7 ± 0.3	7.5 × 10²
5	23.8 ± 3.9	19 ± 2.1	8.0 × 10²	< 0.50^c	12 ± 0.7	> 2.4 × 10⁴	3.6 ± 1.3	3.5 ± 0.4	9.9 × 10²

The K_m and k_cat could not be determined due to the linear relationship at the concentration range (5.2-333 μM) used, but k_cat/K_m can be obtained from the slope of the linear plot (Figure S1a).

The value is 51 μM for SIRT2 and 323 μM for SIRT3 provided in the commercial assay kit.

The K_m could not be determined due to the detection limit of the HPLC assay. The lowest substrate concentration used was 0.5 μM.