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. Author manuscript; available in PMC: 2017 Feb 21.
Published in final edited form as: Org Biomol Chem. 2016 Jan 21;14(7):2186–2190. doi: 10.1039/c5ob02609a

Table 2.

Evaluation of the fluorogenic assay by measuring signal-to-background (S/B) ratio and Z′-factor at various substrate concentrations.

Substrate 4 concentration (μM)
2.5 5 10 20
SIRT1 S/B 21 75 48 30
Z′-factor -0.45 0.98 0.83 0.68
SIRT2 S/B 60 165 80 45
Z′-factor 0.85 0.87 0.72 0.93
SIRT3 S/B 14 45 38 16
Z′-factor 0.61 0.92 0.89 0.81