Fig. 3. Autophagy contributed to cell death induced by OA in HepG2 cells.

(A) HepG2 cells were treated with 30, 100 µM OA for 12 h in the presence or absence of 5 mM 3-MA or 50 µM CQ, LC3 expression were detected by western blot. (B) HepG2 cells were treated with 100 µM OA for 12 h in the presence or absence of 5 mM 3-MA for 2 h and then stained with MDC. Scale bar: 5 µm. (C) HepG2 cells were treated with various concentrations of OA and/or 1 or 5 mM 3-MA for 24 h. Cell death was measured by MTT assay. (D) SMC7721 cells were treated with various concentrations of OA and/or 1 or 5 mM 3-MA for 24 h. Cell death was measured by MTT assay. ^*p<0.05, ^**p<0.01. The results shown are representative of three different experiments.