Fig. 4. Attenuating effect of EA on the gelatinolytic activity of promatrix metalloproteinase-2 (proMMP-2) in the conditioned media from human dermal fibroblasts under UV-B irradiation.

Fibroblasts were subjected to fresh media with the varying concentrations (0, 5, 12 or 30 µmol/L) of EA for 30 min before irradiation. In A, the proMMP-2 gelatinolytic activity in conditioned medium was detected using gelatin zymography. The relative band strength was determined with densitometry using ImageJ software. In B, the equal loading of conditioned media was shown by the silver staining. Data are presented as % of control versus the non-irradiated control (Mean±SD, n=3). ^*p<0.05 versus the non-treated control (UV-B irradiation alone).