Fig. 7. Enhancing effect of EA on the Nrf2 levels in human dermal fibroblasts under UV-B irradiation.

Fibroblasts were subjected to fresh media with the varying concentrations (0, 5, 12 or 30 µmol/L) of EA for 30 min before the irradiation. The Nrf2 proteins were determined using western blotting analysis with anti-Nrf2 antibodies. GAPDH was used as a protein loading control. The relative band strength was determined with densitometry using ImageJ software. Data are presented as % of control versus the non-irradiated control (Mean±SD, n=3). ^##p<0.01 versus non-irradiated control; ^**p<0.01 versus non-treated control (UV-B irradiation alone).