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. 2016 May 9;7:119. doi: 10.3389/fphar.2016.00119

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Copyright © 2016 Sasagawa, Nishimura, Hayakawa, Murakami, Ashikawa, Yuge, Okabe, Kawaguchi, Kawase and Tanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recovery of zebrafish locomotive behavior after SCI was inhibited by treatment with E2F4 inhibitor. (A) Protocol for the assessment of zebrafish locomotive behavior. Zebrafish were placed in each well of a 48-well-plate. The plate was set in a device to monitor the behavior of zebrafish in real-time. In the device, the plate was illuminated for 30 min for acclimatization, followed by five sets of dark (3 min) and light (3 min) periods. Zebrafish with SCI (B–D) or without SCI (E–G) were treated with an E2F4 inhibitor HLM006474 (5 μM) or with vehicle. The distance moved during the base (30 min, B,E), dark periods (15 min, C,F) or light periods (15 min, D,G) are shown. N = 36 for each group with SCI and eight for each group without SCI. ^#p < 0.05 compared to SCI without HLM006474.