Figure 3.

(A) Effects of glutamate on NSC-34_D cell cultured in DMEM/Ham’s F12 without RA. Cells were exposed to increasing concentrations of glutamate (ranging from 0.1 to 10 mM) for 48 h before counting. (B) Effects of glutamate on survival of primary MN, cells were exposed at 8 DIV to 0.1 mM glutamate for 48 h before counting. Data were analyzed by Mann-Whitney test or Kruskal-Wallis (multiple comparisons) to detect statistical significance. Statistically significant difference from untreated cells *p < 0.05, **p < 0.01. Data are mean ± SEM (n = 3).