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. 2016 May 9;10:118. doi: 10.3389/fncel.2016.00118

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Copyright © 2016 Madji Hounoum, Vourc’h, Felix, Corcia, Patin, Guéguinou, Potier-Cartereau, Vandier, Raoul, Andres, Mavel and Blasco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acute effects of 100 μM and 1 mM glutamate (for primary MN and NSC-34_D, respectively) for 200 s on calcium entry. Representative fluorescence measurement of Ca²⁺ entry in MN (A) and NSC-34_D (B) after acute application of glutamate. Glutamate was added at the time indicated by the arrow. (A) Mean traces of cytosolic Ca²⁺ flux of MN and (B) NSC-34_D in response to glutamate. (C) Histograms showing maximum fluorescence intensity following glutamate application in MN and NSC-34_D. Mean data ± SEM from three coverslips per cell type with at least five cells per coverslip analyzed. *p < 0.001 (Mann-Whitney test MN vs. NSC-34_D).