FIGURE 3.

H₂O₂ accumulation and lipid peroxidation in response to brassinosteroid and/or zinc oxide nanoparticles (ZnO NPs) treatments. (A) In situ detection of H₂O₂ using H2DCF-DA staining, Bar = 200 μm, (B) H₂O₂ contents, and (C) malondialdehyde (MDA) contents in seedling roots after 15 days culture in the ½ MS medium containing 10 and 50 mg/L ZnO NPs with or without 5 nM 24-epibrassinolide (BR). The data are the means of four replicates, with standard errors indicated by the vertical bars. Means denoted by the same letter did not significantly differ at a P < 0.05, according to Tukey’s test. CK, control; FW, fresh weight; NP10, 10 mg/L ZnO NPs; NP50, 50 mg/L ZnO NPs.