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. 2016 May 9;7:688. doi: 10.3389/fmicb.2016.00688

FIGURE 4.

FIGURE 4

nrdJ and nrdD expression in P. aeruginosa wild-type, PW3784 Δanr, PW1965 Δdnr and PW7549 ΔnarL strains. GFP fluorescence of (A) PnrdJ and PnrdJ ΔAnr/Dnr-box and (B) of PnrdD and PnrdD ΔAnr/Dnr-box measured in the wild-type, PW3784 Δanr, PW1965 Δdnr and PW7549 ΔnarL strains at a mid-logarithmic phase after 3 h of anaerobic induction. A fragment of the sequence of the corresponding promoter regions surrounding the putative Anr/Dnr-box is added at the right, and the sequence of the box is indicated in bold letters. In the mutant Anr/Dnr-boxes, mutated nucleotides are indicated in capital letters and underlined. The Anr/Dnr box is centered at -84 and -98 bp from the translation start site of the nrdJ and nrdD genes, respectively. ∗Significantly different from the wild-type strain in an unpaired t-test (P < 0.05). Error bars represent the standard deviation for three independent experiments.