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. 2016 May 9;7:682. doi: 10.3389/fmicb.2016.00682

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Copyright © 2016 Bergs, Ishitsuka, Evangelinos, Nienhaus and Takeshita.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of actin cables with cell end marker or microtubules. (A) Wide-field image sequences of an actin cable by GFP-TpmA. The plus end, visualized by GFP-TpmA, moved along the apical membrane. Arrows indicate the plus end of the actin cable moving along the apical membrane. The elapsed time is given in seconds. Scale bar: 2 μm. (B) The strain expressing Lifeact-GFP under the alcA promoter and mRFP1-TeaA under the native promoter was grown in 2% glycerol containing medium. Scale bar: 2 μm. (C,D) Localization of actin cables by Lifeact-GFP and microtubules by mCherry-TubA. Super-resolution microscopy image was constructed by Airyscan (ZEISS; D). Scale bars: 2 μm.