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. 2016 May 9;7:643. doi: 10.3389/fmicb.2016.00643

Figure 5.

Figure 5

Detection and identification of P. salmonis in different fish tissues and species. (A) DNA extracted from P. salmonis LF-89 infected Salmo salar tissues were used to perform PCR-RFLP assays using PmaCI in early or late state of fish infection. (Upper panel) PCR amplification of Salmo salar 60S rDNA gene (Ss60sS27F/Ss60s27R), (Middle panel) 16S rDNA PCR amplification (using primers 27F/1492R) and (Lower panel) PCR-RFLP digestion pattern using enzyme PmaCI. In all cases: 1 and 4, head kidney samples; 2 and 5, spleen samples; 3 and 6, brain samples; M, O'GeneRuler 100 bp DNA Ladder Plus. (B) DNA extracted from P. salmonis LF-89 infected tissues from 1, Oncorhynchus tshawytscha (CHSE-214 embrionic cells); 2, Oncorhynchus mykiss (head kidney); 3, Oncorhynchus kisutch (head kidney), were used to perform PCR-RFLP assay using restriction enzyme PmaCI. (Upper panel) Genomic DNA visualized in a 2% agarose gel electrophoresis, (Middle panel) 16S rDNA amplicon (using primers 27F/1492R) visualized in a 2% agarose gel electrophoresis, and (Lower panel) PCR-RFLP digestion pattern visualized in a 2% agarose gel electrophoresis. M, O'GeneRuler 100 bp DNA Ladder Plus.