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. 2016 Mar;6(1):82–92. doi: 10.1086/685023

Figure 2.

Figure 2

Cytochrome P450 1B1 (CYP1B1) expression is increased in female SERT+ mice, and CYP1B1 inhibition increases estrogen concentrations in lung homogenates. Female SERT+ mice were injected with 1.5 mg/kg 2,3′,4,5′-tetramethoxystilbene (TMS) by intraperitoneal injection for 2 weeks and compared with wild-type (WT; C57Bl/6J CBA) and SERT+ vehicle-treated mice. A, Whole-lung homogenates (30 μg) were analyzed for CYP1B1 expression by Western blotting and normalized to GAPDH; n = 5–8. B, Representative CYP1B1 immunolocalization in pulmonary arteries, as determined by VIP peroxidase–positive cells (pink). Scale bar: 20 μm. C, Estrogen (17β-estradiol) concentrations as determined by enzyme-linked immunosorbent assay in 400 μg of whole-lung homogenates; n = 4–5. Data were analyzed by a 1-way analysis of variance followed by a Tukey post hoc test. ***P < 0.001. SERT+: serotonin transporter is overexpressed.