Figure 3.

SERT⁺ mice have increased RNA and DNA oxidative damage in the lungs. A, Lung sections obtained from wild-type (WT) and SERT⁺ mice and SERT⁺ mice treated with the cytochrome P450 1B1 (CYP1B1) inhibitor 2,3′,4,5′-tetramethoxystilbene (TMS) were immunostained for the oxidative-stress marker 8-hydroxyguanosine (8-OHG). Sections were counterstained with the nuclear stain DAPI (4′,6-diamidino-2-phenylindole). Scale bar: 50 μm. B, 8-OHG mean fluorescence intensity was measured in whole-lung sections with ImageJ software; n = 4–5. Data were analyzed by a 1-way analysis of variance followed by a Tukey post hoc test. *P < 0.05; **P < 0.01. SERT⁺: serotonin transporter is overexpressed.