Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 9;6:25463. doi: 10.1038/srep25463

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Experimental binding curves for (A) galectin-1 and (B) galectin-3 to asialofetuin (ASF), equine synovial fluid FPLC-purified lubricin, and equine synovial fluid FPLC-purified lubricin +0.1 M β-lactose. Galectin-3 binds to lubricin with high affinity, and this interaction is inhibited by the addition of 0.1 M β-lactose, indicative of carbohydrate-dependent binding. (C,D) Binding curves for (C) galectin-1 and (D) galectin-3 to native lubricin vs. sialidase-treated lubricin. (E) K_d values for galectin binding to ASF and lubricin. Calculations were performed by non-linear curve fitting with Python. The errors of the fitted Kds are the square root of variances returned by the fitting algorithm.