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. 2016 May 9;6:25654. doi: 10.1038/srep25654

Figure 1. Property of recombinant proteins and growth curve of the recombinant strains.

Figure 1

(A) The molecular weights of Cel3A, CelA and Cel7A with (+) or without the PNGase F treatment. For deglycosylation treatment, 48 μL denatured protein with or without 2 μL PNGase F enzyme solution (500 U/mL) was incubated in 50 mM sodium phosphate (pH 7.5) at 37 °C for 2 h. (B) The extracellular activity of Cel3A in recombinant strains. (C) The extracellular activity of CelA in recombinant strains. (D) The extracellular activity of Cel7A in recombinant strains. (E) The molecular weight of secreted Cel3A. (F) The growth curve of wild-type and N-glycosyaltion defective mutants in YPD medium. WT: wild-type strain expressing recombinant protein, och1Δ: OCH1 deletion strain expressing recombinant protein, mnn1Δ: MNN1 deletion strain expressing recombinant protein, mnn9Δ: MNN9 deletion strain expressing recombinant protein. The data are presented as the means ± standard errors from three independent experiments.