Figure 2. The effect of deglycosylation enzymes to the molecular weight and activity of the recombinant proteins.

(A) The molecular weight of extracellular Cel3A with or without deglycoslylation enzyme treatment. (B) The ratio of extracellular Cel3A activity with or without Endo F3 treatment. Compared with the control, Endo F3 removed the N-glycans efficiently, thus the activity of Cel3A with and without deglycosylation by Endo F3 was measured. (C) The molecular weight of extracellular CelA with or without deglycosylation enzyme treatment. (D) The ratio of extracellular CelA activity with or without Endo F1 treatment. Compared with the control, Endo F1 removed the N-glycans efficiently, thus the activity of CelA with and without deglycosylation by Endo F1 was measured. (E) The molecular weight of extracellular Cel7A activity with or without deglycosylation enzyme treatment. (F) The ratio of extracellular Cel7A activity with or without Endo F1 treatment. Compared with the control, Endo F1 removed the N-glycans efficiently, thus the activity of Cel7A with and without deglycosylation by Endo F1 was measured. Control: samples without deglycosylation enzyme treatment. PNGase F: samples with PNGase F treatment; Endo F 1/2/3: samples with Endo F1, Endo F2 or Endo F3 treatment, respectively. The data are presented as the means ± standard errors from three independent experiments.