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(A) The mean percent and standard deviation of β-hematin formed was measured using a range of detergent concentrations. The optimal detergent concentration of Triton X-100 detergent was found to be 30 μM, the lowest concentration at which maximum β-hematin was observed, determined by a multiple comparison one-way ANOVA test. (B) The optimal detergent concentration of NP-40 and the Triton X detergents varies with respect to the PEO side chain length (n).
