Figure 7.
(A) The amount of solubilized heme was quantified after 10 min for NP-40 and the TXn detergents using the pyridine-ferrochrome method. The amount of solubilized heme increased in a sigmoidal pattern with increasing PEO lengths. Measurements were taken in triplicate with reported standard deviations. (B) TXn-mediated β-hematin formation follows a sigmoidal growth pattern. The value of n is indicated for each of the kinetic growth curves. (C) The t1/2 of β-hematin formation was determined for each of the TXn detergents, with a shorter chain length resulting in a longer half-life. (D) The percentage of heme crystallization was determined after 120 min of incubation with the TXn detergents (circles) revealing a similar pattern to the amount of heme solubilized by the detergent mediators. NP-40 (triangle) resulted in much faster kinetics with a greater amount of crystallized heme formed at 120 min compared to all other detergents. Measurements were taken in triplicate with reported standard deviations.