Table 1. Optimal Detergent Concentration, the Minimum Concentration That Promotes Maximal β-Hematin Formation, Was Determined for Each of the Detergents along with the Yield of β-Hematin Formation Using a Multiple Comparison One-Way ANOVA to Evaluate Significancea.
detergent name | abbreviation | optimal concentration (μM) | % β-hematin formation |
---|---|---|---|
lipid blend | SNLDs | 50 | 89 |
Tween 20 | 10 | 87 | |
Tween 80 | 15 | 82 | |
sodium dodecyl sulfate | SDS | 1140 | 40 |
(3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) | CHAPS | 1465 | 51 |
Nonidet P-40 | NP-40 | 30 | 88 |
Triton X-45 | TX4.5 | 50 | 86 |
Triton X-114 | TX7.5 | 40 | 80 |
Triton X-100 | TX9.5 | 30 | 80 |
Triton X-102 | TX12 | 20 | 80 |
Triton X-165 | TX16 | 10 | 76 |
Triton X-305 | TX30 | 5 | 79 |
The detergents were solubilized in water and incubated at 37 °C while shaking for 24 h.