Table 3. Best-Fit Value of the Avrami Equation Was Determined for Each of the Detergentsa.
detergent name | n | r2 | z (min–n)b |
---|---|---|---|
Tween 20 | 1 | 0.94 | 0.12 ± 0.02 |
Tween 80 | 1 | 0.96 | 0.11 ± 0.01 |
NP-40c | 4 | 0.97 | 7.26 ± 1.40 × 10–8 |
Triton X-45 | 4 | 0.99 | 7.52 ± 0.57 × 10–12 |
Triton X-114c | 4 | 0.97 | 2.68 ± 0.34 × 10–10 |
Triton X-100 | 4 | 0.99 | 9.65 ± 0.77 × 10–10 |
Triton X-102 | 4 | 0.99 | 1.87 ± 0.13 × 10–9 |
Triton X-165 | 4 | 0.98 | 4.92 ± 0.52 × 10–9 |
Triton X-305 | 4 | 0.99 | 9.11 ± 0.95 × 10–9 |
The kinetics of β-hematin formation were analyzed following incubation of a mixture of heme (50 μM) and detergent (50 μM) at 37 °C and pH 4.8 while shaking with the mean and standard deviations of three replicates reported.
The rate constant calculated based on the indicated Avrami constant, n.
Triton X-114 and NP-40 had a slightly better fit for n = 3.