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. 2016 May 9;6:25673. doi: 10.1038/srep25673

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) A schematic illustration of the generation of bone marrow chimeric mice. Whole bone marrow was harvested from a green fluorescence protein (GFP) transgenic mouse donor. Bone marrow cell transplantation was performed, and the reconstituted bone marrow chimeric mice were analyzed at eight weeks after transplantation. (B) The immunohistochemical analysis of the injured spinal cord at 12 hours after SCI with GFP (green), PMN (red), Iba1 (white) and Hoechst (blue) staining. Almost all of the infiltrating peripheral blood-derived cells were PMN-positive neutrophils. (C) An immunohistochemical analysis of the injured spinal cord at 12 hours after SCI with laminin (light blue), GFP (green), PMN (red) and Hoechst (blue) staining. The lower images are magnifications of the boxed areas in the upper images. Neutrophils were observed inside the laminin-positive blood vessel walls. Scale bars (B): 100 μm; (C): 200 μm; inset: 50 μm.